Journal: Scientific reports

Article Title: Increased primary breast tumor expression of CD73 is associated with development of bone metastases and is a potential biomarker for adjuvant bisphosphonate use.

doi: 10.1038/s41598-025-92841-9

Figure Lengend Snippet: Fig. 1. Representative images of immunostaining for CD73 in TMA cores from patients in the AZURE study. (A) Images of protein expression obtained using the anti-CD73 antibody and visualized at a magnification of 20x. Score 0–3 categories were determined by the intensity of staining in the cytoplasmic compartment of tumor cells only. Scale bar = 200 μm. (B) 40x magnification view of from the boxed regions depicted in panel A. Scale bar of core view = 100 μm. Scale bar of 40x = 50 μm.

Article Snippet: After cooling and washing, slides were blocked with normal goat serum for 1 h at room temperature, after which primary antibodies against CD73 (CST, 13160, 1:1000) were applied (overnight, 4 °C).

Techniques: Immunostaining, Expressing, Staining

Journal: Scientific reports

Article Title: Increased primary breast tumor expression of CD73 is associated with development of bone metastases and is a potential biomarker for adjuvant bisphosphonate use.

doi: 10.1038/s41598-025-92841-9

Figure Lengend Snippet: Fig. 2. Prognostic value of CD73 levels in clinical events. Kaplan-Meier association of CD73 expression level in Overall Survival (OS) in (A) control and (B) zoledronic acid arms. Relationship between level of expression of CD73 in Disease-Free-Survival (DFS) in (C) control and (D) zoledronic acid arms. P-value is obtained from the log-rank test for testing quality of survival Q15 functions.

Article Snippet: After cooling and washing, slides were blocked with normal goat serum for 1 h at room temperature, after which primary antibodies against CD73 (CST, 13160, 1:1000) were applied (overnight, 4 °C).

Techniques: Expressing, Control

Journal: Scientific reports

Article Title: Increased primary breast tumor expression of CD73 is associated with development of bone metastases and is a potential biomarker for adjuvant bisphosphonate use.

doi: 10.1038/s41598-025-92841-9

Figure Lengend Snippet: Fig. 3. Kaplan-Meier association of CD73 levels with clinical metastatic events. Relationship between CD73 expression to time to first bone recurrence, – where cancer spread to bone is the first recorded event in (A) control and (B) zoledronic acid arm. Relationship between level of CD73 expression to spread to bone at any time (for which patients may have had spread to non-bone sites first) in (C) control and (D) zoledronic acid arm. Relationship between CD73 expression to time to spread to non-bone sites in (E) control and (F) zoledronic acid arm. P-value is obtained from the log-rank test for testing quality of survival Q15 function.

Article Snippet: After cooling and washing, slides were blocked with normal goat serum for 1 h at room temperature, after which primary antibodies against CD73 (CST, 13160, 1:1000) were applied (overnight, 4 °C).

Techniques: Expressing, Control

Journal: Journal of Cancer Research and Clinical Oncology

Article Title: Effect of human bone marrow mesenchymal stem cell-derived microvesicles on the apoptosis of the multiple myeloma cell line U266

doi: 10.1007/s00432-024-05822-2

Figure Lengend Snippet: Western blotting assay for the verification of the BMSC-MVs. CD63, CD73, and CD81 with approximately 50, 75, and 26 kDa protein bands, respectively, were detected

Article Snippet: Next, the membranes were separately incubated with the following primary antibodies: rabbit polyclonal anti-human CD63 antibodies (cat. No. 11,271-T16; Sino Biological, China), rabbit monoclonal anti-human CD73 antibodies (cat No. ab124725; Abcam, MA, United States), and rabbit polyclonal anti-human CD81 antibodies (cat No. ab155760; Abcam, MA, United States) as per the suppliers’ instructions.

Techniques: Western Blot